Research Associate I / II - Research Associate - Cell therapy / Marson Lab
Company: Gladstone Institutes
Location: San Francisco
Posted on: October 18, 2024
Job Description:
Research Associate I / II - Research Associate - Cell therapy /
Marson Lab
About the role:
The Gladstone-UCSF Institute of Genomic Immunology is recruiting a
Research Associate (RA) to help perform studies related to genetic
engineering and functional assessment of T cell therapies,
primarily CAR-T cells. Our institute has pioneered the application
of novel genetic engineering technologies such as CRISPR to primary
human immune cells. These new technical abilities have furthered
our research into the genetic control of T cell biology as well as
opened the door to novel cancer immunotherapies based on engineered
human immune cells. This individual would be part of a preclinical
research team under the direction of Justin Eyquem (UCSF)
streamlining the generation, benchmarking, and functional
assessment of genetically engineered human T cell therapies for
cancer and inflammatory disease.
What you will do:
- The RA will utilize various protocols to generate the molecular
components, such as DNA constructs, gRNA library, lentiviral
vectors and Adeno-Associated Vectors necessary for specific
cellular engineering experiments.
- Isolation and culture of primary human T cells, as well as
functional analysis of engineered cells via flow cytometry,
cellular assays, Sanger sequencing, next-generation DNA sequencing,
Single-Cell RNA sequencing.
- Design and completion of cellular editing experiments, as well
as in vitro analysis of novel cellular cancer immunotherapies and
coordination with the in vivo team for mouse experiments employing
the T cell products under development.
What you will need:
- BS or BA or MS 2+ years related experience in cellular Biology,
immunology, or related field; or an equivalent combination of
education and experience.
- New graduates with a commitment to learning are welcome to
apply.
- Experience with general laboratory techniques, especially basic
molecular and cellular biology techniques (including but not
limited to DNA and RNA isolation, PCR, gel electrophoresis, DNA
assembly, bacterial transformation, mammalian cell culture,
etc.).
- Experience with T cell engineering using viral and non-viral
gene editing technologies, including retroviral vectors, AAV,
RNA/RNP electroporation and analysis via multicolor flow
cytometry.
- Experience with in vitro characterization of engineered T cells
to evaluate cytotoxicity, proliferation, cytokine production and
phenotype.
- Experience with animal studies, including basic animal care,
tumor monitor and assessment is a plus.
- Experience with library design, screening and NGS sequencing is
a plus.
- Excellent attention to detail, organizational and interpersonal
communication skills (verbal and written).
- Willingness and ability to learn new methods and proactive at
troubleshooting and optimization.
- Ability to work independently and as a member of a research
team.
- Ability to prioritize tasks, coordinate work tasks with others,
and meet multiple deadlines.
- Ability to comply with policies and procedures.
- Some evening and weekend hours are required.
What Gladstone will offer you:
- People -work with talented, committed, and supportive teammates
within an organization that values each member of its
community.
- A meaningful place to grow and learn -whether it is your
professional skills or scientific knowledge, we have the resources
and environment to advance either.
- Healthy work/life balance -you are highly engaged and
productive at work because you can have time to recharge and enjoy
a vibrant life outside of work.
- Compensation -competitive salary. Title and salary will be
commensurate with education and experience.
- Excellent benefits -generous medical, dental, vision,
retirement plan, paid vacation, commuter benefits, access to free
shuttle transportation.
If this role is not for you:
Please stay in touch and to see a complete list of our open career
opportunities, please visit:
Hiring Range:
- RA I $25-$29/hr.
- RA II $66,560- $78,306K
Gladstone Perks & Benefits:
- People -work with talented, committed, and supportive teammates
within an organization that values each member of its
community.
- A meaningful place to grow and learn -whether it's your
professional skills or scientific knowledge, we have the resources
and environment to advance either.
- Healthy work/life balance -you are highly engaged and
productive at work because you can have time to recharge and enjoy
a vibrant life outside of work.
- Compensation -competitive salary. Title and salary will be
commensurate with education and experience.
- Excellent benefits -generous medical, dental, vision,
retirement plan, paid vacation, commuter benefits, access to free
shuttle transportation.
Gladstone is committed to providing equal employment opportunities
to all employees and applicants for employment without regard to
race, color, sex, religion, national origin, ancestry, age, marital
status, medical condition, physical or mental disability, veteran
status, sexual orientation, or any other non-job related
characteristic. We make all employment decisions so as to further
this principle of equal employment.
About Us
Based in San Francisco's Mission Bay neighborhood, Gladstone is an
independent state-of-the-art biomedical research institution that
empowers its world-class scientists to find new pathways to
cures.
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Keywords: Gladstone Institutes, Fremont , Research Associate I / II - Research Associate - Cell therapy / Marson Lab, Healthcare , San Francisco, California
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